Genetic Variation of Golden Silk-Producing Insect Cricula trifenestrata Helf. (Lepidoptera: Saturniidae) Using ISSR (Inter Simple Sequence Repeats) Molecular Marker

Agnes Herlina Dwi Hadiyanti(1*),


(1) Universitas Sanata Dharma
(*) Corresponding Author

Abstract


The aims of this research were to conduct a study on genetic variation C. trifenestrata collected from several sampling locations using ISSR (Inter-Simple Sequence Repeat) molecular markers. The applied methods of the research were to collect samples (C. trifenestrata), isolation DNA genome, and analysis of genetic variation using the ISSR-PCR method. Based on genetic variation analysis using 4 primers (ISSR 1, ISSR 2, ISSR 6, dan ISSR 7), it is known that the level of genetic variation and polymorphism of C. trifenestrata is high with a percentage of 98.9%. The formed dendogram showed that the 29 studied samples of C. trifenestrata separate into two major groups at the similarity level of (0,60) 60%.


Keywords


Genetic variation Cricula trifenestrata; ISSR Molecular Marker

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References


Agisimanto, D., Supriyanto, A. (2007). Keragaman genetik pamelo Indonesia berdasarkan Primer Random Amplified Polymorphic DNA. J. Hort. 17 (1), 1-7. DOI: 10.21082/jhort.v17n1.2007.p%25p

Akai, H. (1997). Recent aspect of wild silkmoth and silk research. In Seminar at Gajah Mada University, Yogyakarta, 16 April 1997. Available Online: https://ci.nii.ac.jp/naid/ 10026886950/

Borror, D.J. (1992). Pengenalan Pelajaran Serangga. Gadjah Mada University Press. Yogyakarta.

Campbell, B. C., LeMare, S., Piperidis, G., Godwin, I. D. (2011). IRAP, a retrotransposon-based marker system for the detection of somaclonal variation in barley. Molecular breeding 27(2), 193-206. DOI: 10.1007/s11032-010-9422-4

Campbell, N. A., Reece, J. B., & Mitchell, L. G. (2002). Biologi jilid 1. Jakarta: Erlangga.

Chatterjee S.N., Vijayan K, Roy G.C., Nair C.V. (2004). ISSR profiling of genetic variability in the ecotypes of Anthereae mylitta drury, the tropical tasar silkworm. Russian Journal of Genetics, 40(2), 152-159. Available online, https://www.ncbi.nlm.nih.gov/pubmed/ 15065428

d'Abrera, B. (1995). Saturniidae mundi=. Automeris Press, in association with Hill House. Available online: http://agris.fao.org/agris-search/search.do?recordID=US201300022733

Frankham R., Ballou, J. D., Briscoe, D.A. (2002). Introduction to Conservation Genetics. Cambridge: Cambridge University Press.

Indrawan M, Primack R. B., Supriatna J. (2007). Biologi Konservasi. Jakarta: Yayasan Obor Indonesia.

Indrawan, M. (2002). Potensi Sutra Liar dalam Pengembangan Industri dan Perlindungan Lingkungan Hidup. Enviro. (1) 2, 22-24.

Isshiki, S., Iwata, N., & Khan, M. M. R. (2008). ISSR variations in eggplant (Solanum melongena L.) and related Solanum species. Scientia Horticulturae, 117(3), 186-190. DOI: 10.1016/j.scienta.2008.04.003

Jakaria, Mansjoer, S.S., Saefuddin, A., Kaomini, M. (2001). Analisis interaksi genotipe-lingkungan pada beberapa sifat kuantitatif ulat sutera (Bombyx mori L.). Med. Pet. 24 (2), 24-29. Available online, http://repository.ipb.ac.id/jspui/bitstream/123456789/ 26120/4/Jakaria_AnalisisInteraksiGenotipe.pdf

Moczek, A.P. (2010). Phenotypic plasticity and diversity in insects, The Royal Society Journal. Phil. 365, 593–603. DOI: 10.1098/rstb.2009.0263

Neog, K., Singh, H. R., Unni, B., Sahu, A. K. (2010). Analysis of genetic diversity of Muga silkworm (Antheraea assamensis, Helfer; Lepidoptera, Saturniidae) using RAPD-based molecular markers. African Journal of Biotechnology. 9(12):1746-1752. DOI: 10.5897/AJB10.1745

Nindhia, T. G. T., Knejzlik, Z., Ruml, T., Nindhia, T.S. (2014). Tensile properties and biocompatibility of Indonesian wild silk Cricula trifenestrata, A Preliminary Study. Journal of Medical and Bioengineering 3 (2), 140-143. DOI: 10.12720/jomb.3.2.140-143

Norbertus K. (2010). Sutra Emas. Surakarta: Penerbit Delta Media

Pharmawati, M. (2009). Marka Molekular Berbasis DNA untuk Penentuan Hubungan Kekerabatan Tumbuhan. Denpasar: Universitas Udayana

Pinratana, B.A, Lampe, R.E.J. (1990). Moths of Thailand. Vol 1. Saturniidae. Bangkok: Bosco Offset. Yannawa

Reddy K.D., Nagaraju J., Abraham E.G. (1999). Genetic characterization of the Silkworm Bombyx mori by Simple Sequence Repeat (SSR)-Anchored PCR. Heredity. 83, 681-687. DOI: 10.1046/j.1365-2540.1999.00607.x

Rucińska, A., & Puchalski, J. (2011). Comparative molecular studies on the genetic diversity of an ex situ garden collection and its source population of the critically endangered polish endemic plant Cochlearia polonica E. Fröhlich. Biodiversity and conservation, 20(2), 401-413. DOI: 10.1007/s10531-010-9965-z

Situmorang, J. (1996). An attempt to produce Attacus atlas (Lepidoptera: Saturniidae) using baringtonia leaves as plant fodder. International journal of wild silkmoth & silk, 2, 55-57. Available online: https://ci.nii.ac.jp/naid/10029602739/

Subositi, D., & Kasiamdari, R. S. (2010). Variasi intraspesifik stevia (Stevia rebaudiana (bertoni) bertoni) koleksi balai besar penelitian dan pengembangan tanaman obat dan obat tradisional (b2p2to-to) berdasarkan karakter morfologi dan molekular [Doctoral Dissertation]. Yogyakarta: Universitas Gadjah Mada. Available online: http://etd.repository.ugm.ac.id/index.php? mod=book_detail⊂=BookDetail&act=view&typ=htmlext&buku_id=48776&obyek_id =4&unitid=1&jenis_id=

Suryanto, D. (2003). Melihat keanekaragaman organisme melalui beberapa teknik genetika molekuler. Medan: Universitas Sumatera Utara

Tikader, A., Vijayan, K., Saratchandra, B., (2014). Cricula trifenestrata (Helfer) (Lepidoptera, Saturniidae) – a silk producing wild insect in India. Trop. Lepid. Res., 24(1), 22-29. Available online: http://journals.fcla.edu/troplep/article/view/89730/86065

Vijayan, K., Anuradha, H. J., Nair, C. V., Pradeep, A. R., Awasthi, A. K.,

Saratchandra, B., ... & Urs, S. R. (2006). Genetic diversity and differentiation among populations of the Indian eri silkworm, Samia cynthia ricini, revealed by ISSR markers. Journal of Insect Science, 6(1), 1-11 DOI: 10.1673/2006_6_30.1

Wahyuni, S., Xu, D.H., Bermawie, N., Tsunematsu, H., Ban, T. (2004). Skrining ISSR Primer Studi Pendahuluan Kekerabatan Antar Jahe Merah, Jahe Emprit dan Jahe Besar. Buletin TRO 15(1), 33-41. DOI: 10.21082/bullittro.v15n1.2004.%25p

Widiastuti, A., Sobir, S., & Suhartanto, M. R (2013). Analisis keragaman genetik manggis (Garcinia mangostana) diiradiasi dengan sinar gamma berdasarkan penanda ISSR. Bioteknologi Biotechnological Studies, 10(1), 15-22. Available online: https://smujo.id/bbs/article/view/1581

Williams, J. G., Kubelik, A. R., Livak, K. J., Rafalski, J. A., & Tingey, S. V. (1990). DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic acids research, 18(22), 6531-6535. Available online, https://www.ncbi.nlm.nih.gov/pubmed/ 1979162

Yulita, K.S. (2013). Keragaman Genetik Beberapa Aksesi Jagung dari Nusa Tenggara Timur Berdasarkan Profil Inter Short Sequence Repeat (ISSR). Jurnal Biologi Indonesia 9(2), 255-263. DOI: 10.14203/jbi.v9i2.171




DOI: http://dx.doi.org/10.24235/sc.educatia.v7i1.2514

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