Genetic Variation of Golden Silk-Producing Insect Cricula trifenestrata Helf. (Lepidoptera: Saturniidae) Using ISSR (Inter Simple Sequence Repeats) Molecular Marker

Agnes Herlina Dwi Hadiyanti

doi:10.24235/sc.educatia.v7i1.2514

Genetic Variation of Golden Silk-Producing Insect Cricula trifenestrata Helf. (Lepidoptera: Saturniidae) Using ISSR (Inter Simple Sequence Repeats) Molecular Marker

Agnes Herlina Dwi Hadiyanti^(1*),

(1) Universitas Sanata Dharma
(*) Corresponding Author

Abstract

The aims of this research were to conduct a study on genetic variation C. trifenestrata collected from several sampling locations using ISSR (Inter-Simple Sequence Repeat) molecular markers. The applied methods of the research were to collect samples (C. trifenestrata), isolation DNA genome, and analysis of genetic variation using the ISSR-PCR method. Based on genetic variation analysis using 4 primers (ISSR 1, ISSR 2, ISSR 6, dan ISSR 7), it is known that the level of genetic variation and polymorphism of C. trifenestrata is high with a percentage of 98.9%. The formed dendogram showed that the 29 studied samples of C. trifenestrata separate into two major groups at the similarity level of (0,60) 60%.

Keywords

Genetic variation Cricula trifenestrata; ISSR Molecular Marker

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DOI: 10.24235/sc.educatia.v7i1.2514